Antigua ‘materia oscura’ microbiana: miles de especies bacterianas desconocidas descubiertas en las cuevas de lava de Hawái

Steve Smith en un pasillo de una cueva hawaiana lleno de raíces del distrito de Kau en la isla de Hawái. Crédito: Kenneth Ingham

Las antiguas cuevas de lava de la isla de Hawái contienen miles de especies bacterianas desconocidas

Se ha descubierto una diversidad bacteriana más alta de lo que esperaban los científicos en cuevas de lava, tubos de lava y respiraderos geotérmicos en la Isla Grande de Hawái. Los resultados se informan en un nuevo estudio publicado hoy (21 de julio de 2023) en la revista Fronteras en Microbiología.

Esta investigación investiga la diversidad y las interacciones dentro de estos ecosistemas microbianos, que explican cómo existe la vida.[{” attribute=””>Mars and the early Earth in the past. Surprisingly, the findings showed that a class of bacteria known as Chloroflexi are often “hub” species, meaning that they are connected with many other species and typically play crucial ecological roles in the community. Many Chloroflexi species are little understood, and further research will reveal previously undiscovered species, as well as insights into the role these species play in these extreme environments.

“This study points to the possibility that more ancient lineages of bacteria, like the phylum Chloroflexi, may have important ecological ‘jobs,’ or roles,” said first author Dr. Rebecca D Prescott of NASA Johnson Space Center and University of Hawaiʻi at Manoa, in the US. “The Chloroflexi are an extremely diverse group of bacteria, with lots of different roles found in lots of different environments, but they are not well studied and so we don’t know what they do in these communities. Some scientists call such groups ‘microbial dark matter’ — the unseen or un-studied microorganisms in nature.”

Unseen volcanic life

Prescott and her colleagues gathered 70 samples from a variety of locations, including active geothermal vents (fumaroles), as well as “younger” and “older” lava tubes and caves, which were under 400 years old and between 500 and 800 years old, respectively, to get a sense of how the bacterial communities might change over time. They were able to determine the diversity and abundance of the bacterial classes in each sample by sequencing the ribosomal RNA present in the samples. Co-occurring bacterial networks also provided hints regarding possible interactions between these microorganisms.

Thick microbial mats hang under a rock ledge in steam vents that run along the Eastern Rift Zone on Hawaiʻi Island. Credit: Jimmy Saw

The harshest conditions—the geothermal sites—were expected to have lower diversity than the more established and habitable lava tubes. While the diversity was indeed found to be lower, the team of researchers was surprised to discover that the interactions within these communities were more complex than in locations with higher diversity.

“This leads to the question, do extreme environments help create more interactive microbial communities, with microorganisms more dependent on each other?” said Prescott. “And if so, what is it about extreme environments that helps to create this?”

Since Chloroflexi, and another class called Acidobacteria, were present at nearly all of the locations, they may play essential roles in these communities. However, these were not the most abundant bacteria, and the individual communities from the different sites showed large variations in the diversity and complexity of the microbial interactions. Counterintuitively, the most abundant groups, Oxyphotobacteria and Actinobacteria, were not often ‘hub’ species, suggesting that their roles may be less important to the overall structure of the community.

More questions than answers

Since the current study was based on the partial sequencing of one gene, it cannot accurately determine the species of microbes or their ‘jobs’ in the community. Therefore, further research is needed to help reveal the individual species that are present, as well as to better understand these bacteria’s roles in the environment.

A stalactite formation in a Hawaiian cave system from this study with copper minerals and white microbial colonies. Despite the fact that copper is toxic to many organisms, this formation hosts a microbial community. Credit: Kenneth Ingham

“Overall, this study helps to illustrate how important it is to study microbes in co-culture, rather than growing them alone (as isolates),” said Prescott. “In the natural world, microbes do not grow in isolation. Instead, they grow, live, and interact with many other microorganisms in a sea of chemical signals from those other microbes. This then can alter their gene expression, affecting what their jobs are in the community.”

Beyond the insights about past, or even future, life on Mars, bacteria from volcanic environments can also be useful in understanding how microbes turn volcanic rock (basalt) into soils, as well as bioremediation, biotechnology, and sustainable resource management.

Reference: “Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles” by Rebecca D. Prescott, Tatyana Zamkovaya, Stuart P. Donachie, Diana E. Northup, Joseph J. Medley, Natalia Monsalve, Jimmy H. Saw, Alan W. Decho, Patrick S. G. Chain and Penelope J. Boston, 21 July 2023, Frontiers in Microbiology.
DOI: 10.3389/fmicb.2022.934708

Funding: NASA Headquarters, George Washington University